解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::In Drosophila, flies with two X chromosomes are females, with one X chromosome, males. We investigated the presence of sex determining factors on the X chromosome by constructing genotypes with one X and various X-chromosomal duplications. We found that female determining factors are not evenly distributed along the X...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90284-3
更新日期:1985-10-01 00:00:00
abstract::The transforming protein of Rous sarcoma virus (pp60v-src) and its normal cellular homolog (pp60c-src) are demonstrated to be phosphorylated at serine 12 in vivo under certain conditions. We propose that protein kinase C is responsible for this modification based on the following evidence. First, the tumor promoters, ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90281-8
更新日期:1985-10-01 00:00:00
abstract::Thrombin stimulates 32Pi incorporation into phosphatidylinositol 4-phosphate (PIP), phosphatidylinositol 4,5-bis-phosphate (PIP2), and phosphatidylinositol (PI), and initiates DNA synthesis in hamster (NIL) fibroblasts at a half-maximal concentration of 125 ng/ml. Neomycin, which binds PIP2 and PIP, inhibits both thro...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90105-9
更新日期:1985-09-01 00:00:00
abstract::Clarification of the information processing system in bacterial sensing has been obtained by studying mutants that lack the capacity to modify receptors covalently. The remaining part of the system is able to receive signals from the receptor, to respond with partial adaptation, and to exhibit a chemotactic response. ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90125-4
更新日期:1985-09-01 00:00:00
abstract::The hsp 70 gene of Drosophila contains three domains to which a heat shock gene specific transcription factor (HSTF) binds. In addition to the previously described 55 bp binding domain proximal to the TAT homology, there are two 25 bp binding sites farther upstream. Footprinting studies with 5' and 3' deletion mutatio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90110-2
更新日期:1985-09-01 00:00:00
abstract::We report that the 12,000 dalton domain of fibronectin that interacts with fibroblast cell surfaces also binds specifically to thrombin-inducible, saturable receptors on platelets. Furthermore, we have used chemical cross-linking and monoclonal antibodies to show that the 12,000 dalton domain of fibronectin interacts ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90101-1
更新日期:1985-09-01 00:00:00
abstract::Trypanosoma brucei variant surface glycoprotein (VSG) genes are activated either by duplicative (DA) transposition of the gene to a pre-activated expression site or by nonduplicative (NDA) activation of a previously silent telomeric gene. We have obtained a recombinant clone spanning the 5' barren region of the expres...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80113-6
更新日期:1985-08-01 00:00:00
abstract::We have determined the complete 9202 nucleotide sequence of the visna lentivirus. The deduced genetic organization most closely resembles that of the AIDS retrovirus in that there is a novel central region separating pol and env. Moreover, there is a close phylogenetic relationship between the conserved reverse transc...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80132-x
更新日期:1985-08-01 00:00:00
abstract::The surface glycoproteins T4 and T8 define different functional subsets of T lymphocytes and may act as recognition molecules mediating appropriate interactions between the T cell and its target. Previously we employed gene transfer and subtractive hybridization to isolate a T8 cDNA; now we have isolated and sequenced...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80105-7
更新日期:1985-08-01 00:00:00
abstract::The location of cis-acting regulatory sequences within the long terminal repeat (LTR) of the human T cell lymphotropic virus type III (HTLV-III/LAV) was determined. An enhancer element capable of increasing the rate of transcription from a heterologous promoter, irrespective of distance and orientation, is located bet...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80062-3
更新日期:1985-07-01 00:00:00
abstract::The subcellular distribution of the type II enzyme of cAMP-dependent protein kinase (cAMP-dPK II) in epithelial and fibroblastic cells was determined by indirect immunofluorescence microscopy. In interphase cells both regulatory (R II) and catalytic (C) subunits were concentrated in a perinuclear area. By comparison o...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80084-2
更新日期:1985-07-01 00:00:00
abstract::Vanadate has been shown to inhibit phosphatases that remove phosphate groups from phosphotyrosine in cell-free systems. Addition of vanadate to the culture medium of NRK-1 cells resulted in a maximal 40-fold increase in the level of phosphotyrosine in cell protein. Also, vanadate induced transformation as evidenced by...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80051-9
更新日期:1985-07-01 00:00:00
abstract::Certain mutant alleles at the low density lipoprotein (LDL) receptor locus produce receptors that bind LDL normally, but fail to cluster in coated pits and therefore cannot transport LDL into cells. We prepared genomic DNA libraries from cells of two individuals with this phenotype (internalization-defective familial ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80054-4
更新日期:1985-07-01 00:00:00
abstract::A strong transcription enhancer was identified in the genomic DNA (235 kb) of human cytomegalovirus (HCMV), a ubiquitous and severe pathogen of the herpesvirus group. Cotransfection of enhancerless SV40 DNA with randomly fragmented HCMV DNA yielded two SV40-HCMV recombinant viruses that had incorporated overlapping se...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80025-8
更新日期:1985-06-01 00:00:00
abstract::We have localized the regulatory sequence required for viral or poly(I)-poly(C) activation of human beta-interferon gene expression to a region located between -37 and -77 from the mRNA cap site. This sequence has the characteristics of an inducible enhancer element: it can act upstream or downstream of the beta-inter...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/s0092-8674(85)80024-6
更新日期:1985-06-01 00:00:00
abstract::We have identified a 2.4 kb partial cDNA clone (pDL34) for human von Willebrand factor (vWf) mRNA. pDL34 was selected by screening an endothelial cell cDNA library with a radiolabeled reverse transcript of mRNA obtained by specific immunoisolation of vWf polysomes from endothelial cells. pDL34 selectively hybridized t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90060-1
更新日期:1985-05-01 00:00:00
abstract::We have shown that lariat formation during in vitro splicing of several RNA precursors, from Drosophila to man, occurs at a unique and identifiable but weakly conserved site, 18 to 37 nucleotides proximal to the 3' splice site. Lariat formation within an artificial intron lacking a normal branch-point sequence occurs ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90064-9
更新日期:1985-05-01 00:00:00
abstract::We have characterized an aberrant allele of a variably spliced Drosophila tropomyosin gene. The allele was recovered from the flightless Ifm(3)3 strain, which has been shown to have structurally and functionally abnormal indirect flight muscles. The transcribed portion of the mutant gene is interrupted by an 8,8 kb in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90061-3
更新日期:1985-05-01 00:00:00
abstract::We have constructed an actin-HIS4 gene fusion, such that expression of HIS4 requires proper splicing of the actin intron. Using this chimeric gene in an in vivo screen for splicing mutations, we have isolated a G to A transition in the fifth position of the yeast 5' consensus sequence/GTAPyGT. This mutation still allo...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90065-0
更新日期:1985-05-01 00:00:00
abstract::We have examined the long-range distribution of double-stranded DNA cleavage sites for Drosophila melanogaster topoisomerase II. These studies reveal a novel partitioning of preferred topoisomerase II cleavage sites. In the eukaryotic DNAs examined, major cleavage sites were typically found in nontranscribed spacer se...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90353-8
更新日期:1985-04-01 00:00:00
abstract::A 1.1 kb intron containing an open reading frame (ORF) in one allele (omega+) of the yeast mitochondrial 21S rRNA gene is nearly quantitatively inserted in crosses into a 21S rRNA allele lacking that intron (omega-). We have determined that this nonreciprocal exchange initiates soon after cells fuse to form zygotes an...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90348-4
更新日期:1985-04-01 00:00:00
abstract::In induced erythroleukemia cells, transcription of the beta-globin gene terminates in a region 600-1500 nucleotides downstream of the poly(A) site. To determine whether this region of the mouse DNA functions to terminate transcription when moved to another genomic site, portions of the putative termination region have...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90349-6
更新日期:1985-04-01 00:00:00
abstract::In many cases the multiple genes coding for one specific tRNA are dispersed throughout the genome. The members of such a gene family nevertheless maintain a common nucleotide sequence during evolution. A major mechanism contributing to this concerted evolution is intergenic conversion. Here we show that it occurs betw...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90347-2
更新日期:1985-04-01 00:00:00
abstract::A clone encoding PrP 27-30, the major protein in purified preparations of scrapie agent, was selected from a scrapie-infected hamster brain cDNA library by oligonucleotide probes corresponding to the N terminus of the protein. Southern blotting with PrP cDNA revealed a single gene with the same restriction patterns in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90333-2
更新日期:1985-04-01 00:00:00
abstract::A transglutaminase-catalyzed cross-linking process characteristic of keratinocytes leads to the formation of the insoluble corneocyte envelope. The essentials of this process take place in vitro in a reconstituted system derived from subcellular fractions. A particulate fraction containing membrane-bound envelope prec...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90216-8
更新日期:1985-03-01 00:00:00
abstract::The v-erbB gene product of avian erythroblastosis virus (AEV) has extensive homology with the receptor for epidermal growth factor (EGF). We report here that chicken embryo fibroblasts (CEF) transformed by AEV show enhanced tyrosine phosphorylation of a number of cellular polypeptides, including the 36 kd protein, whi...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90209-0
更新日期:1985-03-01 00:00:00
abstract::DNA repair was measured in the dihydrofolate reductase gene in Chinese hamster ovary cells, amplified for the gene, by quantitating pyrimidine dimers with a specific UV-endonuclease. More than two thirds of the dimers had been removed from a 14.1 kb restriction fragment of the gene by 26 hr after irradiation (20 J/m2)...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90150-3
更新日期:1985-02-01 00:00:00
abstract::The achaete-scute gene complex (AS-C), involved in differentiation of the sensory chaetes of D. melanogaster, and the yellow locus have been cloned. The yellow locus is the most distal and is followed, proximally, by the achaete and the scute loci. In the scute locus (75 kb), three transcription units separated by lon...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90147-3
更新日期:1985-02-01 00:00:00
abstract::Chimeric histocompatibility genes encoding the amino-terminal (beta 1) domain of the class II Ak beta polypeptide and the carboxy-terminal (C2, transmembrane, and intracytoplasmic) domains of either the class I H-2Ld or H-2Dd molecules were stably introduced into mouse L cells. Although both were transcribed, only 5' ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90139-4
更新日期:1985-02-01 00:00:00
abstract::S. cerevisiae strains containing RAS2val19, a RAS2 gene with a missense mutation analogous to one that activates the transforming potential of mammalian ras genes, have growth and biochemical properties strikingly similar to yeast strains carrying IAC or bcy1. Yeast strains carrying the IAC mutation have elevated leve...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90305-8
更新日期:1985-01-01 00:00:00
abstract::We have performed in vitro kinetic analyses of transcription from the 5S gene promoter in order to resolve the rate-limiting events which lead to accurate transcription of the 5S RNA gene. We demonstrate that a rate-limiting intermediate can be formed during an extended incubation prior to initiation of transcription....
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(85)90315-0
更新日期:1985-01-01 00:00:00
abstract::Using a cloned cDNA complementary to a portion of the mRNA for the 50 kd human epidermal keratin, we have screened a human genomic library and have isolated and sequenced the gene encoding this keratin. A comparison of the keratin gene with the very distantly related vimentin gene has enabled us to explore the relatio...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90456-2
更新日期:1984-12-01 00:00:00
abstract::We have used liposomes to deliver DNAase I inside normal Syrian hamster embryo (SHE) cells. We showed the entrance of DNAase I inside the cell by dose-dependent cytotoxicity; and the entrance of DNAase I into the nucleus by the induction of chromosomal aberrations and somatic mutation at the HPRT locus (but not at the...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90450-1
更新日期:1984-12-01 00:00:00
abstract::A unique DNA structural intermediate associated with termination of replication in Bacillus subtilis has been identified. This was achieved by probing for the Bam HI DNA fragment within which the terminus of the chromosome (terC) resides. The intermediate migrates more slowly than the bulk of digested chromosomal DNA ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90475-6
更新日期:1984-12-01 00:00:00
abstract::Using a combination of classical genetics, serology, and molecular genetics, we have mapped as many as possible of the MHC components in t haplotypes. Results indicate that the whole MHC is included in a simple inversion with the relative positions of its genes being intact. Furthermore, some of the t-lethal mutations...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90464-1
更新日期:1984-12-01 00:00:00
abstract::Treatment of guinea pig neutrophils with pertussis toxin (islet-activating protein; IAP) results in inhibition of N-formyl peptide receptor-mediated release of arachidonic acid and granular enzymes. Inhibition by the toxin is specific, in that responses to the calcium ionophore A23187 are not affected. The action of t...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90008-4
更新日期:1984-12-01 00:00:00
abstract::The kinetics of association of epidermal growth factor (EGF) and diferric-transferrin (TF) with clathrin-coated membranes of KB cells were examined using anti-clathrin antibody bound to Staphylococcus aureus. The ligands were bound to cells at 4 degrees C and after warming to 30 degrees C for 5 min, both ligands were ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90006-0
更新日期:1984-12-01 00:00:00
abstract::We have prepared polyclonal antibodies to the cytoplasmic portion of the envelope glycoprotein G of vesicular stomatitis virus (VSV) by using synthetic peptides corresponding to either the 22 or 11 ultimate carboxy-terminal residues of the G as immunogens. When antibodies to the 22 residue peptide are microinjected in...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90195-8
更新日期:1984-11-01 00:00:00
abstract::We present evidence that Tn10 transposase promotes double strand breaks and single strand joints at Tn10 termini in vivo. Plasmids containing a shortened Tn10 element and a transposase overproducer fusion give rise, upon transposase induction, to new DNA species. The most prominent class is a circularized transposon m...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90204-6
更新日期:1984-11-01 00:00:00
abstract::We examine the interaction between polyoma-virus-encoded middle tumor antigen and the cellular src gene product, pp60c-src, using a series of monoclonal antibodies that recognize mammalian pp60c-src. Our results show that infection of mouse cells with transformation-competent strains of polyoma virus results in the st...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(84)90272-1
更新日期:1984-10-01 00:00:00